Re: Fourteen Babies Born after Round Spermatid Injection into Human Oocytes
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منابع مشابه
Fourteen babies born after round spermatid injection into human oocytes.
During the human in vitro fertilization procedure in the assisted reproductive technology, intracytoplasmic sperm injection is routinely used to inject a spermatozoon or a less mature elongating spermatid into the oocyte. In some infertile men, round spermatids (haploid male germ cells that have completed meiosis) are the most mature cells visible during testicular biopsy. The microsurgical inj...
متن کاملRound spermatid injection.
This finding has caused great concern with round spermatid enthusiasts. A major complaint is that “one should not restrict the label of round spermatid only to those cells in which an acrosomal vesicle is visible” (p. 2977) (4). However, our observation is based not only on phase-contrast evaluation of TESE microdroplets but also on detailed stained histology of many hundreds of testis specimen...
متن کاملSuccessful fertilization and pregnancy after injection of frozen-thawed round spermatids into human oocytes.
The effect of cryopreservation on the integrity and fertilizing capacity of round spermatids was studied in two azoospermic patients. In December 1995 the patients, both with maturation arrest of spermatogenesis, were submitted to testicular sperm extraction (TESE) after an extensive examination of their ejaculate. Only round spermatids were found after testicular biopsy. Some of the spermatids...
متن کاملSpermatid injection into human oocytes. I. Laboratory techniques and special features of zygote development.
Spermatid injection into the oocyte cytoplasm has been shown recently to yield viable human embryos developing to term after transfer to the mother. This study provides details of the laboratory techniques related to round spermatid injection (ROSI) and elongated spermatid injection (ELSI) and focuses on some special features of zygote development associated with the use of these types of sperm...
متن کاملSuccessful pregnancy after spermatid injection.
We present nine cases of spermatid intracytoplasmic injection for the treatment of non-obstructive azoospermia. In eight cases, no elongated spermatids or spermatozoa were found in previous spermiograms or testicular biopsies. In these patients, treatment was performed using ejaculated (n = 6) and testicular (n = 2) retrieved round spermatids (Sa type). In cases where ejaculated round spermatid...
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ژورنال
عنوان ژورنال: Journal of Urological Surgery
سال: 2016
ISSN: 2148-9580
DOI: 10.4274/jus.2016.02.012